Secretory Capacity of Isolated Pancreatic Acinar Cells

Assessing the secretory capacity of pancreatic acinar cells.

Pancreatic acinar cells produce and secrete digestive enzymes. These cells are organized as a cluster which forms and shares a joint lumen. This work demonstrates how the secretory capacity of these cells can be assessed by culture of isolated acini. The setup is advantageous since isolated acini, which retain many characteristics of the intact exocrine pancreas can be manipulated and monitored...

Internalization of cationized ferritin by isolated pancreatic acinar cells.

The internalization of cationized ferritin (CF) was studied in isolated pancreatic acinar cells in vitro. Horseradish peroxidase (HRP) was used in conjunction with CF to compare internalization of soluble-phase and membrane-bound tracers. The mode of internalization of CF was dependent upon tracer concentration and origin of the plasma membrane (apical vs. lateral-basal). At the lower tracer co...

Pancreatic Acinar Cells

Insulin protects pancreatic acinar cells

Background: Impaired metabolism and cytosolic Ca overload in pancreatic acinar cells can trigger pancreatitis. Results: Insulin protected cells from oxidantinduced Ca overload, inhibition of the plasma membrane calcium pump (PMCA) and ATP depletion. Conclusion: Insulin switches metabolism towards glycolysis and fuels the PMCA even when mitochondria are impaired. Significance: This mechanism may...

Trisphosphate in Pancreatic Acinar Cells

The mechanisms of agonist-induced Ca 2 1 spikes have been investigated using a caged inositol 1,4,5-trisphosphate (IP 3 ) and a low-affinity Ca 2 1 indicator, BTC, in pancreatic acinar cells. Rapid photolysis of caged IP 3 was able to reproduce acetylcholine (ACh)induced three forms of Ca 2 1 spikes: local Ca 2 1 spikes and submicromolar ( , 1 m M) and micromolar (1–15 m M) global Ca 2 1 spikes...